Home > Sandwich ELISA Kits > Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

Product Name:

Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

Cat.#:

JL11358

Regulatory Status:

RUO

Alternative:

CXCL11; I-TAC; IP9; SCYB11; SCYB9B; B-R1; Chemokine(C-X-C-Motif)ligand 11; Small Inducible Cytokine Subfamily B Member 11; Interferon gamma-inducible protein 9

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

18.93pg/mL

Specificity:

This kit recognizes Human ITaCin samples. No significant cross-reactivity or interference between Human ITaC and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| ITaC

C-X-C motif chemokine 11 (CXCL11) is a protein that is encoded by the CXCL11 gene.C-X-C motif chemokine 11 is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant (I-TAC) and Interferon-gamma-inducible protein 9 (IP-9). It is highly expressed in peripheral blood leukocytes, pancreas and liver, with moderate levels in thymus, spleen and lung and low expression levels were in small intestine, placenta and prostate.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.15	1.7	1.2	0.77	0.43	0.23	0.14	0.04
Corrected OD	2.11	1.66	1.16	0.73	0.39	0.19	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human ITaC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	93-105	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human ITaC and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	87-96	90-110
1 : 2	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	104-115
1 : 4	Average (%)	94	98	108